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IIT Bombay Study Identifies Gentler Enzyme to Retrieve Lab-Grown T Cells for Cancer Therapy

Using Accutase preserved cell viability with intact surface proteins during detachment from dense fibre scaffolds.

Overview

  • The peer-reviewed work, conducted with Monash University’s Prof. Neil Cameron, appears in Biomaterials Science.
  • Researchers cultured Jurkat T cells in polycaprolactone electrospun scaffolds, where cells lodged tightly at fibre junctions and resisted manual flushing.
  • Accutase outperformed TrypLE and flushing by maintaining higher survival and more normal immune behavior, including post-recovery clustering and growth.
  • Scaffold-grown T cells showed stronger in‑vitro cancer cell killing than cells expanded on flat dishes, indicating a dual benefit of the approach.
  • The team plans animal studies next and is exploring implantable, T‑cell‑loaded scaffolds to advance adoptive T‑cell therapies such as CAR T.