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Hidden Filament Architecture in Cellular Condensates Is Essential for Function

A Scripps Research team used cryo-electron tomography to map PopZ scaffolds, tying internal architecture to condensate function.

Overview

  • Media reports detail a February 2 Nature Structural & Molecular Biology study showing that some biomolecular condensates contain ordered filament networks rather than being simple liquids.
  • Cryo-electron tomography revealed PopZ assembles through a stepwise process into thin filaments that form a scaffold dictating condensate physical properties.
  • Single-molecule FRET showed PopZ adopts distinct conformations inside versus outside condensates, linking protein shape to location within the droplet.
  • A filament-deficient PopZ mutant produced more fluid condensates with lower surface tension and caused halted growth and failed DNA segregation in bacteria.
  • The findings suggest new therapeutic strategies that act on condensate architecture in diseases such as cancer and ALS, which researchers describe as a developing direction.